Diversity of Apple mosaic virus Isolates in India Based on Coat Protein and Movement Protein Genes
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Diversity of Apple mosaic virus Isolates in India Based on Coat
Protein and Movement Protein Genes
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| Creator |
Lakshmi, Vijay
Hallan, Vipin Raja, Ram Ahmed, N Zaidi , A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
Abstract Apple mosaic virus (ApMV), an Ilarvirus is one
of the most common pathogens of apple worldwide. During
field surveys in commercial plantations of Himachal Pradesh
and Jammu & Kashmir, observations of bright chlorotic
mosaic like symptoms on apple trees indicated
probable infection by the virus, which was later detected by
double antibody sandwich-enzyme linked immunosorbent
assay (DAS-ELISA). An incidence of 24 and 28% (based
on ELISA) was obtained as 6/25 and 15/53 samples from
HP and J&K were positive, respectively. An amplification
of approximately 700 and 850 bp was obtained for coat and
movement protein genes (CP and MP), respectively. The
CP was 223 amino acids in length and showed 87–99%
identity when compared to 21 ApMV isolates. Whereas,
MP (286 amino acids) showed 91–95% identity with other
isolates. However, the gene sequences were quite conserved
among Indian isolates and grouped together phylogenetically.
CP of the Indian isolates showed maximum
identity of 95% with Korean isolate (AY 125977) in apple
and in other host these showed a maximum identity of 98%
to Czech Republic pear isolate. MP showed maximum
identity with Chinese isolate i.e., 95%. The diversity study
will also help in analyzing variability among the isolates
and also to formulate diagnostic and resistance strategies.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/938/1/30%2D01%2D2012_Diversity.pdf
Lakshmi, Vijay and Hallan, Vipin and Raja, Ram and Ahmed, N and Zaidi , A A (2011) Diversity of Apple mosaic virus Isolates in India Based on Coat Protein and Movement Protein Genes. Indian Journal of Virol, 22 (1). pp. 44-49. |
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| Relation |
http://ihbt.csircentral.net/938/
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