An improved method for thin layer chromatographic analysis of saponins
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
An improved method for thin layer chromatographic analysis of saponins
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| Creator |
Sharma, O P
Kumar, Neeraj Bhat., Tej. K Singh , Bikram |
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| Subject |
Plant sciences
Natural Product Chemistry |
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| Description |
a b s t r a c t
Analysis of saponins by thin layer chromatography (TLC) is reported. The solvent system was n-butanol:
water:acetic acid (84:14:7). Detection of saponins on the TLC plates after development and air-drying
was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood
on the plate surface. Saponins appeared as white spots against a pink background. The protocol provided
specific detection of saponins in the saponins enriched extracts from Aesculus indica (Wall. ex Camb.)
Hook.f., Lonicera japonica Thunb., Silene inflata Sm., Sapindus mukorossi Gaertn., Chlorophytum borivilianum
Santapau & Fernandes, Asparagus adscendens Roxb., Asparagus racemosus Willd., Agave americana L.,
Camellia sinensis [L.] O. Kuntze. The protocol is convenient, inexpensive, does not require any corrosive
chemicals and provides specific detection of saponins
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/963/1/Food_Chem._2011.pdf
Sharma, O P and Kumar, Neeraj and Bhat., Tej. K and Singh , Bikram (2012) An improved method for thin layer chromatographic analysis of saponins. Journal of Food Chemistry, 132. pp. 671-674. |
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| Relation |
http://ihbt.csircentral.net/963/
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