EDTA Inhibits Peroxidase-Catalyzed Iodide Oxidation Through Interaction at the Iodide Binding Site
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
EDTA Inhibits Peroxidase-Catalyzed Iodide Oxidation
Through Interaction at the Iodide Binding Site
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| Creator |
Banerjee, Ranajit K
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| Subject |
Cell Biology & Physiology
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| Description |
EDTA inhibits the fonnutinn of !~" from iodide ettxiysnd by various pure peroxidases. The inhibition is concentration-
dependent, and chloruperuxidase (CPO) is more sensitive than horseradish peruxidase (HRP) and lactoperuxldase
0LPO). EDTA is more active than EGTA or other biological chelators tasted. Zn 2+, Mn z+ and Co 2+ are equally active
in revet~ing the effect of EDTA on both CPO and EIRP almost completely, bet ineffective in the case of LPO. The
effect of EDTA on HRP can be reversed by a higher concentration of iodide but not by HzO z. EDTA causes a
hypsodwemic change in the absorl~Oa of the So~t band of HRP at 402 nm, and iodide can reverse this effect, EDTA
can effectively displace radioindide specifically bound to HRP. It is suggested that EDTA inhibits iodide oxidation by
interacting at the iodide binding site of the HRP.
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1381/1/BBA_Volume_992___Issue_3___Pages_393%2D396%2C1989[16].pdf
Banerjee, Ranajit K (1989) EDTA Inhibits Peroxidase-Catalyzed Iodide Oxidation Through Interaction at the Iodide Binding Site. Biochimica et Biophysica Acta, 992 (3). pp. 393-396. |
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| Relation |
http://dx.doi.org/10.1016/0304-4165(89)90102-5
http://www.eprints.iicb.res.in/1381/ |
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