Stimulation of Forward Motility of Goat Cauda Epididymal Spermatozoa by a Serum Glycoprotein Factor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Stimulation of Forward Motility of Goat Cauda Epididymal
Spermatozoa by a Serum Glycoprotein Factor
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| Creator |
Mandal, Mahitosh
Banerjee, Saswati Majumder, Gopal Chandra |
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| Subject |
Cell Biology & Physiology
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| Description |
Blood sera of humans, rats, goats, and buffalo have been shown to possess a forward motility-stimulating factor (FMSF) that markedly sti,nula:ed goat cauda epididymal sperm forward motility, as assayed by a microscopic
method in the presence of epididymal plasma (1.2mg protein/mI) that had sufficient anti-sticking activity to
eliminate the possibility of cell-sticking artifacts in motility assays. The spec (fic activity of FMSF was greatest in buffalo blood serwn compared to the sera of the other species. Buffalo serum at a concentration as low as 8.5 mg protein/mi induced forward motility in nearly 45% of the cells. The buffalo serum FMSF was heat-stable, nondialyzable,and sensitive to the action of trypsin. Purified proteins - casein, serum albumin, oval bwnin, myoglobin, and [3-lactoglobulin - showed little or relatively low FMSF activity. FMSF is a glycoprotein, as it binds with high affinity to concanavalin A-agarose. A major portion of the serum protein (approx. 70%) did not bind to the affinity matrix, and this unretained serum protein fraction showed little FMSF activity. The FMSF activity of
buffalo serum was confirmed by estimating sperm forward motility specrophotome:ricaliy: an objective method
of assessing sperm motility.
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1392/1/BIOLOGY_OF_REPRODUCTION__Volume_41___Issue_6___Pages_983%2D989_.1989[4].pdf
Mandal, Mahitosh and Banerjee, Saswati and Majumder, Gopal Chandra (1989) Stimulation of Forward Motility of Goat Cauda Epididymal Spermatozoa by a Serum Glycoprotein Factor. BIOLOGY OF REPRODUCTION, 41 (6). pp. 983-989. |
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| Relation |
http://dx.doi.org/10.1095/biolreprod41.6.983
http://www.eprints.iicb.res.in/1392/ |
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