A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C
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| Creator |
Sonawane, Vijay Chintaman
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| Subject |
QR Microbiology
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| Description |
A mini-scale method for the estimation of D-amino acid oxidase activity against cephalosporin C was developed. The method allows implementation of the oxygenation and thorough mixing in a microfuge tube scale reactor. Dilution of reaction products in a buffer containing 0.03% hydrogen peroxide converted the intermediate product of the enzymatic reaction, α-keto adipyl-7-aminocephalosopranic acid (KA-7-ACA) to glutaryl-7-aminocephalosporanic acid (GL-7-ACA). In combination with high performance liquid chromatography, D-amino acid oxidase activity in the whole cells of Trigonopsis variabilis could be measured based on cephalosporin consumed in the reaction or the GL-7-ACA formed. This method could also be used for assay of D-amino acid oxidase activity in crude and soluble preparations
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| Publisher |
Taylor and francis group
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1080/10242420500132417
http://crdd.osdd.net/open/996/ |
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| Identifier |
Sonawane, Vijay Chintaman (2005) A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C. Biocatalysis and Biotransformation, 23 (2). pp. 117-121. ISSN 1024-2422
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