The N-Terminal of a Heparin-Binding Sperm Membrane Mitogen Possess Lectin-like Sequence
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
The N-Terminal of a Heparin-Binding Sperm Membrane Mitogen Possess Lectin-like Sequence
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| Creator |
Mor, Visesato
Chatterjee, Tapati |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Glycosaminoglycans like heparin and heparin sulfate in follicular fluid induce changes in the intracellular environment during the
spermatozoal functional maturation. We previously reported the isolation, purification and partial characterization of a heparin binding
sperm membrane protein (HBSM). In the present study, the amino acids analysis provided evidence of a single sequence, which suggest
the homogeneity of the purified HBSM. Fourteen amino acids—1A D T I V A V E L D T Y P N14—correspond to the amino terminal
sequence of Concanavalin A (Con A) and contain 45.2% carbohydrate by weight. HBSM possess mitogenic property on lymphocytes
with comparable magnitude to the well-known mitogen; Con A, inducing 83% radiolabel thymidine incorporation in growing lymphocytes.
Unlike Con A, there was no agglutination of cell by HBSM upto 5 ng/ml concentration. Interestingly, we found that heparin and
chondroitin sulfate-conjugated HBSM inhibit the proliferative activity. Similar effect was also found with an in-house isolate sulfated
glycans; G-I (28% sulfate). In contrast, there was no inhibition by the desulfated form; G-ID. Altogether, our data suggest that the mechanism
of cell proliferative pathway may be different for HBSM and Con A.
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| Publisher |
Elsevier
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1417/1/JOURNAL_OF_ETHNOPHARMACOLOGY__110(_2)_189%2D199;2007[104].pdf
Mor, Visesato and Chatterjee, Tapati (2007) The N-Terminal of a Heparin-Binding Sperm Membrane Mitogen Possess Lectin-like Sequence. Biochemical and Biophysical Research Communications, 110 (2). pp. 189-199. |
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| Relation |
http://dx.doi.org/10.1016/j.bbrc.2006.12.196
http://www.eprints.iicb.res.in/1417/ |
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