Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Evaluation of clonal fidelity of in vitro raised plants of
Guadua angustifolia Kunth using DNA-based markers
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| Creator |
Nadha , Harleen kaur
Kumar, Rahul Sharma, R K Anand, Manju Sood, A |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
Guadua angustifolia Kunth a large and multipurpose bamboo has extensive utility in pharmaceutical,
paper, charcoal, and construction industries. Therefore, it is pertinent to scale up their production
through micropropagation technique. This would enable us to meet the growing demand for quality
planting material. Although, the common practice to use axillary bud method allows large-scale
production, there are always possibilities of somaclonal variations which appear in in vitro cultures due
to its rapid multiplication. Therefore, we utilized random amplified polymorphic DNA (RAPD) and intersimple
sequence repeat (ISSR) markers in in vitro raised Guadua clones to assure their genetic fidelity.
We screened 30 RAPD and 27 ISSR primers, and found 15 RAPD and 17 ISSR markers to produce clear,
reproducible and scorable bands. We found 15 RAPD primers which produced 84 distinct bands with an
average of 5.6 bands per primer. In addition, we found 17 ISSR primers which produced 61 distinct
bands in the size range of 300 to 2500 bp. All banding profiles from micropropagated plants were
monomorphic and similar to those of the mother plant, thus ascertaining the true nature of the in vitro
raised plants.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1063/1/101_Evaulation_2011.pdf
Nadha , Harleen kaur and Kumar, Rahul and Sharma, R K and Anand, Manju and Sood, A (2011) Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers. Journal of Medicinal Plants Research, 5 (23). pp. 5636-5641. |
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| Relation |
http://ihbt.csircentral.net/1063/
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