Influence of Human Lactoferrin Expression on Iron Homeostasis, Flavonoids, and Antioxidants in Transgenic Tobacco
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Influence of Human Lactoferrin Expression on Iron Homeostasis,
Flavonoids, and Antioxidants in Transgenic Tobacco
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| Creator |
kumar, Vinay
Gill, T Grover, Sunita Ahuja, Paramvir Singh Yadav , S K |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
This study was aimed at to check the influence
of human lactoferrin (hLF) expression on iron homeostasis,
flavonoids, and antioxidants in transgenic tobacco. Transgenic
tobacco expressing hLF cDNA under the control of a
CaMV 35S promoter was produced. The iron content as
well as chlorophyll content of transgenic tobacco was
lower compared to mock and untransformed wild plants.
Interestingly, hLF transgenic tobacco showed higher level
of transcript expression for genes related to iron content
regulation like iron transporter and metal transporter.
While expression of genes related to iron storage such as
ferritin 1 and ferritin 2 was downregulated. The transcript
expression of genes encoding antioxidant enzymes such as
glutathione reductase, glutathione-S-transferase, ascorbate
peroxidase, and catalase was downregulated in hLF transgenic
tobacco compared to controls. Further, the transcript
expression of two important genes encoding dihydroflavonol
reductase (DFR) and phenylalanine ammonia lyase
regulatory enzymes of flavonoid biosynthesis pathway was
analyzed. The expression of DFR was found to be downregulated,
while PAL expression was upregulated in hLF
transgenic tobacco compared to mock and untransformed
wild plant. Total phenolics, flavonoids, and proanthocyanidins
contents were found to be higher in hLF transgenic
tobacco than the mock and untransformed wild plant
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1083/1/119_Influence_26%2D04%2D2012.pdf
kumar, Vinay and Gill, T and Grover, Sunita and Ahuja, Paramvir Singh and Yadav , S K (2012) Influence of Human Lactoferrin Expression on Iron Homeostasis, Flavonoids, and Antioxidants in Transgenic Tobacco. Mol Biotechnol. |
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| Relation |
http://ihbt.csircentral.net/1083/
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