Lectin-Binding Assay by Polyethylene Glycol 8000
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Lectin-Binding Assay by Polyethylene Glycol 8000
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| Creator |
Abidi, Fatima E
Bishayee, Subal Bachhawat, Bimal K Bhadra, Ranjan |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A quantitative lectin-binding assay using a precipitation technique and polyethylene glycol
X000 (PEG) as a precipitating agent has been described. Carcinoscorpin. a sialic acid-binding
lectin Isolated from the hemolymph of Indian horseshoe crab, C’urc,irfo~l.c,rl,ilr.c ro~w~k~~ comb
and iodinated fetuin. a sialoglycoprotein. were appropriately incubated as the components of
the binding assay. The specific interaction between these two components developed the lectin-
glycoprotein-bound complex. This was subsequently precipitated by the addition of PEG
together with a coprecipitant ~-globulin. Radioactivity of the precipitated bound complex was
estimated to quantify the binding. The formation ofthe bound complex was etfectivcly inhibited
hy a specific sialodisaccharide. O-(Iv:-acetylneuraminyl)-(2 + h)-2-acetumido-2-deoxvgalactitol.
implying the speciftc interaction for such precipitation. The probable effect of PEG was
to stabilize the bound complex. precipitating it along with added y-globulin. This was further
r%ident from the prevention of dissociation of the bound complex and increased binding of
glycoprotein to the imobiivcd lectin in the presence of PEG. The assay was also applicable to
other jialoglycoproteins such as cu,-acid glycoprotein and human chorionic gonadotropin.
Morecvcr, the methcd yielded a saturation plateau with a characteristic hyperbolic binding
curcc. The assay was simple. quich. safe. economic. and highly sensiti\r. L’ 19x7 4cadcmx Press. lnc
KF\~ WOR~IS: lectin (carcinoscorpin): binding assay: polyethy~lrne glyc
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| Publisher |
Elsevier
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1422/1/ANALYTICAL_BIOCHEMISTRY__166(_2)257%2D266;1987[9].pdf
Abidi, Fatima E and Bishayee, Subal and Bachhawat, Bimal K and Bhadra, Ranjan (1987) Lectin-Binding Assay by Polyethylene Glycol 8000. Analytical Biochemistry, 166 (2). pp. 257-266. |
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| Relation |
http://dx.doi.org/10.1016/0003-2697(87)90573-2
http://www.eprints.iicb.res.in/1422/ |
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