Characterization of Vibrio eltor Typing Phages: Properties of the Eltor Phage e4
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Characterization of Vibrio eltor Typing Phages: Properties of the Eltor Phage e4
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| Creator |
Chattopadhyay, Samit
Kinchington, Derek Ghosh, Ranajit K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Biophysical characteristics of Vibrio eltor phage e4, a key phage in the Vibrio cholerae
typing scheme were studied. This icosahedral phage was found to contain 12 structural
polypeptides with mol. wt. ranging from 25 000 to 120000. One of these polypeptides of
mol. wt. 50000 accounted for most of the structural proteins present and was probably
the major phage capsid protein. The phage genome comprised a single linear, doublestranded
DNA molecule, 69.2 kbp in length (45.6 × 106 mol. wt.) as determined by
electron microscopy and restriction fragment analyses. The G + C content was 34.6~.
Electron microscopy data indicated that unlike the DNAs of other cholera phages,
phage e4 DNA is not circularly permuted. Adsorption under normal conditions was
biphasic with rate constants of 1.02 x 10-9/ml/min up to 60~ adsorption and
3 x 10-1°/ml/min thereafter. Intracellular phage multiplication was characterized by a
latent period of 27 min. The burst size was approximately 100 phage particles per
infected cell
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| Publisher |
Society for General Microbiology
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1433/1/JOURNAL_OF_GENERAL_VIROLOGY_(_68)_1411%2D1416[41].pdf
Chattopadhyay, Samit and Kinchington, Derek and Ghosh, Ranajit K (1987) Characterization of Vibrio eltor Typing Phages: Properties of the Eltor Phage e4. Journal of General Virology, 68. pp. 1411-1416. ISSN 0022-1317 |
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| Relation |
http://dx.doi.org/10.1099/0022-1317-68-5-1411
http://www.eprints.iicb.res.in/1433/ |
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