Development and characterization of five monoclonal antibodies against neuronal cell surface antigens - Evaluation of their use in cell separation by affinity chromatography.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Development and characterization of five monoclonal antibodies against neuronal cell surface antigens - Evaluation of their use in cell separation by affinity chromatography.
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| Creator |
Chatterjee, D
Mandal, C Sarkar, P K |
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| Subject |
Cell Biology & Physiology
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| Description |
Five monoclonal antibodies directed against rat neuronal cell surface proteins
have been isolated and characterized following fusion of mouse SP2/O-Ag-14
myeloma cells and spleen cells from BALB/c mice immunized with neuron-specific
membrane proteins. The antibodies displayed cell specificity in binding and elicited
precipitation of different polypeptides from total neuronal cell surface proteins.
These antibodies are of potential use in the isolation of a neuronal subpopulation by
immunoaffinity chromatography. The work represents the application of hybridoma
technology for the isolation of specific types of neuronal populations.
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| Publisher |
Elsevier
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1435/1/JOURNAL_OF_NEUROIMMUNOLOGY__15_(3)_251%2D262[35].pdf
Chatterjee, D and Mandal, C and Sarkar, P K (1987) Development and characterization of five monoclonal antibodies against neuronal cell surface antigens - Evaluation of their use in cell separation by affinity chromatography. Journal of Neuroimmunology, 15 (3). pp. 251-262. |
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| Relation |
http://dx.doi.org/10.1016/0165-5728(87)90120-2
http://www.eprints.iicb.res.in/1435/ |
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