Effect of Erythropoietin on Membrane Lipid Peroxidation, Superoxide Dismutase, Catalase, and Glutathione Peroxidase of Rat RBC
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Effect of Erythropoietin on Membrane Lipid Peroxidation, Superoxide Dismutase, Catalase, and Glutathione Peroxidase of Rat RBC
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| Creator |
Chakraborty, MunMun
Ghosal, Jharna Biswas, Tuli Datta, Asoke G |
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| Subject |
Cell Biology & Physiology
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| Description |
Like many other biological membranes, RBC membranes are prone to lipid
peroxidation, which involves cleavage of polyunsaturated fatty acids at their
double bonds leading to the formation of malonyldialdehyde (1,2). Depletion of
phospholipids in RBC membrane after peroxidant injury (3,4) increases membrane
bilayer rigidity (5-7) and decreases the activities of Na+ + K’ ATPase (8) and
acetylcholinesterase (9,lO). Changes in lipid composition (11) and Na+ + K+
ATPase and acetylcholinesterase of RBC membrane (12) and permeability of
glucose across red cell membrane (13) in response to erythropoietin have been
reported from our laboratory. As red cells are always under high oxygen pressure,
superoxide is continuously being produced within the red cells due to autoxidation
of oxyhemoglobin to met-hemoglobin (14,15). The hydroxyl radicals generated
by the interaction of superoxide with peroxides (16) are always a great threat
to the RBC membrane. The oxidative insult may also cause denaturation and
precipitation of hemoglobin as Heinz bodies (17-19) and these Heinz bodies
remain attached to the RBC membrane by hydrophobic interactions (20,21). The
protective system in red cells against these peroxidative reactions include superoxide
dismutase, catalase, glutathione peroxidase, and glutathione reductase (22-24).
The present study is aimed to study the effect of erythropoietin depletion by
starvation and subsequent replenishment by exogenous administration of erythropoietin
on lipid peroxidation and the protective systems stated above with a
view to evaluating the role of erythropoietin against the peroxidative insults in
RBC.
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1442/1/BIOCHEMICAL_MEDICINE_AND_METABOLIC_BIOLOGY__Volume_40___Issue_1___Pages_8%2D18_%2C1988[30].pdf
Chakraborty, MunMun and Ghosal, Jharna and Biswas, Tuli and Datta, Asoke G (1988) Effect of Erythropoietin on Membrane Lipid Peroxidation, Superoxide Dismutase, Catalase, and Glutathione Peroxidase of Rat RBC. Biochemical Medicine and Metabolic Biology, 40 (1). 08-18. |
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| Relation |
http://dx.doi.org/10.1016/0885-4505(88)90099-0
http://www.eprints.iicb.res.in/1442/ |
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