Purification and Characterization of an Agglutinin from Mucus of the Snail Achatina Fulica
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Purification and Characterization of an Agglutinin from Mucus of the Snail Achatina Fulica
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| Creator |
Mitra, Debashis
Sarkar, Manju Allen, Anthony K |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The mucus of the snail Achatina fulica shows the presence of an agglutinin that nonspecifically
agglutinates human erythrocytes. The agglutinin has been purified by affinity chromatography
using Sepharose 4B-hog gastric mucin as the affinity matrix. Homogeneity was checked by polyacrylamide
gel electrophoresis, immunodiffusion, immunoelectrophoresis, and gel filtration. The agglutinin
is a glycoprotein of native molecular weight 70,000. The isoelectric point of the protein was found to
be 8.0. The predominant amino acids are aspartic acid and glutamic acid (or amides) and serine, which
account for 32% of the total amino acid residues. The agglutinin has 10% carbohydrate (wt/wt) and
the most abundant sugar is N-acetylglucosamine. The cd spectra of the agglutinin show the presence of
random coil conformation. The inhibition of hemagglutination data indicates that the agglutinin is specific
for/3 glycosides of D-Gal and D-GalNAc
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| Publisher |
Elsevier
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1444/1/BIOCHIMIE__Volume_70___Issue_12___Pages_1821%2D1829__%2C1988[2].pdf
Mitra, Debashis and Sarkar, Manju and Allen, Anthony K (1988) Purification and Characterization of an Agglutinin from Mucus of the Snail Achatina Fulica. Biochimie, 70 (12). pp. 1821-1829. |
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| Relation |
http://dx.doi.org/10.1016/0300-9084(88)90044-2
http://www.eprints.iicb.res.in/1444/ |
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