Phospholipids from. the hepatopancreas of Indian horseshoe crab Carcinoscorpius rotundicauda
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Phospholipids from. the hepatopancreas of Indian
horseshoe crab Carcinoscorpius rotundicauda
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| Creator |
Bandyopadhyay, Rajyasree
Basu, Mukul K |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Total phospholipids were extracted from the heart, hepatopancreas, and hemolymph of
the Indian horseshoe crab Carcinoscorpius rotundicauda by the conventional method. Characteristic
group reaction and 2-dimensional thin-layer chromatography on silica gel were used for identification
of different phospholipids. The phospholipid profile obtained from hemolymph and 2 major organs are
comparable and show phosphatidyl choline (PC) and phosphatidyl ethanolamine to be the major
phospholipids. A phospholipid has been consistently detected migrating immediately below the PC in
the thin-layer chromatogram of lipids extracted from the hepatopancreas. When mixed methyl esters
of this slower moving PC are resolved on a silica gel plate ran in hexane ether:acetic acid 80:20:1, with
appropriate controls, an additional spot is seen just below the normal methyl ester, indicating a difference
between the fatty acid compositions of 2 PC (e.g., regular and slower). The slower mixed methyl
esters were found to comprise mainly the 4 saturated fatty acids: lauric, myristic, palmitic, and stearic.
The slow moving PC seems to consist mainly of molecular species with the above-mentioned saturated
fatty acids at both Sn 1 and Sn 2 positions.
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| Publisher |
Elsevier
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1445/1/BIOCHIMIE__Volume_70___Issue_12___Pages_1841%2D1847_%2C1988[3].pdf
Bandyopadhyay, Rajyasree and Basu, Mukul K (1988) Phospholipids from. the hepatopancreas of Indian horseshoe crab Carcinoscorpius rotundicauda. Biochimie, 70 (1). pp. 1841-1847. |
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| Relation |
http://dx.doi.org/10.1016/0300-9084(88)90046-6
http://www.eprints.iicb.res.in/1445/ |
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