Leishmania Donovani : Superoxide Dismutase Level in Infected Macrophages
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Leishmania Donovani : Superoxide Dismutase Level in Infected Macrophages
|
|
| Creator |
Mukherjee, Subrata
Bandyapadhyay, Rajyasree Basu, Mukul K |
|
| Subject |
Drug Development/Diagnostics & Biotechnology
|
|
| Description |
Superoxide dismutase (SOD), a metal containing enzyme is present in parasite Leishmania donovani
as well as in host macrophages both resident and activated in a detectable amount, although the level
is much higher in the latter case. It is observed that at any particular protein concentration, the SOD
activity is highest in the case of parasite infected macrophages and lowest in the case of normal
resident macrophages; the SOD activity of thioglycolate activated macrophages lies in between the
two. It is also noticed that formalin-killed Leishmania donovani neither attach to macrophages nor do
they increase the SOD activity of the host. Thus, the processes, e.g. attachment of the parasite to the
host membrane, subsequent membrane perturbation and thus activation of membrane bound enzyme
NADPH oxidase leading to respiratory burst, may be responsible for an enormous increase in the
SOD level in macrophages during infection. Moreover, the chemical nature of the SOD found in
infected macrophages has been investigated by using an inhibitor, e.g. NaCN, which specifically
inhibits Cu-Zn SOD but not Fe-SOD. A considerable inhibition of SOD activity by NaCN in
infected macrophages confirms the chemical nature of the increased SOD to be of Cu-Zn type,
usually found in host. Presumably, Cu-Zn SOD or host SOD plays a protective role at the time of
parasite infection although the role of parasitic SOD or some other mechanisms for the survival of the
parasite within the toxic phagolysosome environment of the macrophage cannot be ruled out.
|
|
| Publisher |
Kluwer
|
|
| Date |
1988
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1446/1/BIOSCIENCE_REPORTS__Volume_8___Issue_2___Pages_131%2D137%2C1988[43].pdf
Mukherjee, Subrata and Bandyapadhyay, Rajyasree and Basu, Mukul K (1988) Leishmania Donovani : Superoxide Dismutase Level in Infected Macrophages. Bioscience Reports, 8 (2). pp. 131-137. |
|
| Relation |
http://dx.doi.org/10.1007/BF01116457
http://www.eprints.iicb.res.in/1446/ |
|