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A Galactose Specific Agglutinin from the Hemolymph of the Snail Achatina Fulica : Purification and Characterization

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title A Galactose Specific Agglutinin from the Hemolymph of the Snail Achatina Fulica : Purification and Characterization
 
Creator Mitra, Debashis
Sarkar, Manju
 
Subject Infectious Diseases and Immunology
 
Description galactose specific agglutinin has been detected in the hemolymph of the snail Achatina furl ca fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be g.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy- D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose. INTRO
 
Date 1988
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1447/1/DEVELOPMENTAL_AND_COMPARATIVE_IMMUNOLOGY__Volume_12___Issue_1___Pages_33%2D42%2C1988[10].pdf
Mitra, Debashis and Sarkar, Manju (1988) A Galactose Specific Agglutinin from the Hemolymph of the Snail Achatina Fulica : Purification and Characterization. Developmental and Comperative Immuniligy, 12 (1). pp. 33-42.
 
Relation http://dx.doi.org/10.1016/0145-305X(88)90022-5
http://www.eprints.iicb.res.in/1447/