Leishmania donovani: Role of Microviscosity of Macrophage Membrane in the Process of Parasite Attachment and Internalization
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Leishmania donovani: Role of Microviscosity of Macrophage
Membrane in the Process of Parasite Attachment and Internalization
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| Creator |
Mukherjee, Subrata
Ghosh, Chandramallika Basu, Mukul K |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Leishmnnia donovani: Role of
microviscosity of macrophage membrane in the process of parasite attachment and internalization.
Experimental Parasitology 66, 18-26. Host macrophage infection by the parasite
Leishmaniu donovani is heterogeneous, but it is not clear which factors are responsible
for parasite recognition within the macrophages. One possible factor may be the alteration
of the microviscosity of the macrophage membrane. This in turn may affect receptor expression
and hence parasite infection. In this paper we describe alteration of the lipid
composition and hence the microviscosity of the macrophage membrane in a controlled
manner using liposome fusion technique. At a higher macrophage membrane microviscosity
a larger number of parasites have been found to adhere to the macrophage surface. However,
the proportion of parasites finally internalized when compared to parasites adhering to
macrophages is inversely correlated with the artificially altered macrophage membrane
microviscosity. The process of endocytosis has been examined in both native and lipid
modified macrophages in the presence of several sugar antagonists. The results indicate (i)
glucose and mannose are specifically involved in the binding process, and (ii) the microviscosity
has a key role in controlling the macrophage parasite interaction. The results obtained
so far support a model of endocytosis where expression of the receptor is a critical initial
process dependent on the microviscosity of the membrane.
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| Publisher |
Elsevier
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1451/1/EXPERIMENTAL_PARASITOLOGY__Volume_66___Issue_1___Pages_18%2D26%2C1988_[37_].pdf
Mukherjee, Subrata and Ghosh, Chandramallika and Basu, Mukul K (1988) Leishmania donovani: Role of Microviscosity of Macrophage Membrane in the Process of Parasite Attachment and Internalization. Experimental Parasitology, 66 (1). pp. 18-26. |
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| Relation |
http://dx.doi.org/10.1016/0014-4894(88)90046-X
http://www.eprints.iicb.res.in/1451/ |
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