Purification of Human Erythrocytes Specific Lectins from Rice Bean, Phaseolus Calcaratus Syn. Vigna Umbellata, by High-Performance Liquid Chromatography
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Purification of Human Erythrocytes Specific Lectins
from Rice Bean, Phaseolus Calcaratus Syn. Vigna
Umbellata, by High-Performance Liquid
Chromatography
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| Creator |
Datta, Pradip K
Basu, Pranab K Datta, Tapash K |
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| Subject |
Chemistry
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| Description |
Two lectins, an N-acetylgalactosamine-binding lectin, lectin-I, which reacts specifically with human
erythrocytes of blood group A, and a galactose-binding lectin, lectin-II, which is specific for
human blood group B erythrocytes, have been isolated and purified from rice bean, Phoseolus calcaratus
syn. Vigna umbellata, by a salt solubility pH-dependent method, chromatofocusing and highperformance
liquid chromatography. The homogeneity of the lectins was determined by liquid chromatography
and polyacrylamide gel electrophoresis. The purified h&in-I of molecular mass 80 000
is possibly composed of two subunits of molecular mass ca. 18 000 and 22 000, respectively, whereas
lectin-II of molecular mass 100 000 appears to be composed of a monomeric protein of molecular mass
25 000. One endogenous lectin-binding protein was also isolated and purified by liquid chromatography.
The endogenous h&in-binding protein of molecular mass 40 000 affects the activity of the
A-group specific lectin more than that of the B-group specific lectin. The endogenous k&in-binding
protein appears to be composed of a monomeric protein of molecular mass 20 000.
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1454/1/JOURNAL_OF_CHROMATOGRAPHY%2DBIOMEDICAL_APPLICATIONS__Volume_431___Issue_1___Pages_37%2D44%2C1988[23].pdf
Datta, Pradip K and Basu, Pranab K and Datta, Tapash K (1988) Purification of Human Erythrocytes Specific Lectins from Rice Bean, Phaseolus Calcaratus Syn. Vigna Umbellata, by High-Performance Liquid Chromatography. Journal of Chromatography, 431 (1). pp. 37-44. |
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| Relation |
http://dx.doi.org/10.1016/S0378-4347(00)83067-4
http://www.eprints.iicb.res.in/1454/ |
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