Simultaneous Quantification of Flavonoids and Biflavonoids in Ginkgo biloba Using RP-HPTLC Densitometry Method
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Simultaneous Quantification of Flavonoids and
Biflavonoids in Ginkgo biloba Using RP-HPTLC
Densitometry Method
|
|
| Creator |
Kaur, P
Chaudhary, A Singh , Bikram Chand, Gopi |
|
| Subject |
Plant sciences
Natural Product Chemistry |
|
| Description |
A reversed-phase high-performance thin-layer chromatography
(RP-HPTLC) method was developed for the determination of
flavonoids (quercetin and kaempferol) and biflavonoids (sciadopitysin,
ginkgetin, and bilobetin) in the aqueous methanolic extract of
Ginkgo biloba. There have been a number of reports on the quantification
of flavonoids in G. biloba using different analytical techniques,
but the reported methods are not suitable for routine analysis
and also for the simultaneous quantification of flavonoids and
biflavonoids in G. biloba. The method employed here used precoated
plates of silica gel 60F254 as the stationary phase with dual-run
acetonitrile–water–methanol–formic acid (20:20:1:0.005, v/v/v/v)
and acetonitrile–water–methanol–formic acid (20:17:1:0.005,
v/v/v/v) as mobile phases with densitometric determination of
flavonoids and biflavonoids at 254 nm in reflection/absorption
mode. The linear regression analysis data for the calibration plots
showed a linear relationship (r2 from 0.9706 to 0.9990). The method
was validated for accuracy, precision, and robustness. The limits of
detection (LOD) and quantification (LOQ) were in the range of
0.12–0.37 μg and 0.60–1.85 μg, respectively, for the analytes. The
method is reproducible and convenient for quantitative analysis of
these compounds in the leaves of G. biloba. This is the first report of
simultaneous densitometry quantification of major flavonoids and
biflavonoids in G. biloba using the newly developed and validated
RP-HPTLC method
|
|
| Date |
2011
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://ihbt.csircentral.net/1092/1/21_simultaneous.pdf
Kaur, P and Chaudhary, A and Singh , Bikram and Chand, Gopi (2011) Simultaneous Quantification of Flavonoids and Biflavonoids in Ginkgo biloba Using RP-HPTLC Densitometry Method. Journal of planar Chromatography, 24 (6). pp. 507-512. |
|
| Relation |
http://ihbt.csircentral.net/1092/
|
|