EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
EDTA-enhanced lead phytoremediation in sunflower
(Helianthus annuus L.) hydroponic culture
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| Creator |
Seth, C S
Misra, V Singh , R R Zolla, L |
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| Subject |
Plant sciences
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| Description |
The aim of the present study was to
investigate the capability of Sunflower (Helianthus
annuus L.) to tolerate and accumulate high amount of
lead (Pb) and propose it for soil phytoremediation. To
this regard, plants were grown in hydroponics and
treated with different Pb concentrations (10 to
160 μM) and a fixed concentration (500 μM) EDTA
(ethylene diamine tetra acetic acid) for 14 and 28 days
(d). Effects on total biomass production, photosynthetic
pigments and protein contents as well as the
quantities of non protein thiols (NP-SH), glutathione
(GSH), phytochelatins (PCs) and activity of glutathione
reductase (GR) were estimated. Results revealed
that roots (575 μg g−1 DW) and shoots (135 μg g−1
DW) accumulated Pb after 28 d of exposure,
however, addition of EDTA enhanced the Pb accumulation
in roots (645 μg g−1 DW) and shoots
(255 μg g−1 DW ). Exposure of Pb (28 d) registered
a significant (P<0.05) reduction in growth parameters
and induction of phytochelatins (P<0.05; r=0.26)
plus some of the important antioxidants (P<0.05; r=
0.42), which were positively correlated to metal
accumulation. Sunflower exposed at 40 μM of Pb
for 28 d synthesized higher quantity of PC2 (18.5
fold) and PC3 (10.5 fold), as compared to control.
However, the results showed that addition of EDTA
resulted in low toxicity compared to Pb alone. These
data support the capability of H. annuus L. to
accumulate and tolerate significant quantity of Pb
and its utility for phytoremediation. This is because of
the plant has the capacity to combat metal induced
oxidative stress via significant synthesis of NP-SH,
GSH and high activity of GR, as it would provide
sufficient GSH not only for PCs synthesis but also for
antioxidant function.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1108/1/39.pdf
Seth, C S and Misra, V and Singh , R R and Zolla, L (2011) EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture. Plant and Soil, 347 (1-2). pp. 231-242. |
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| Relation |
http://ihbt.csircentral.net/1108/
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