The use of Nano-Quercetin to Arrest Mitochondrial Damage and MMP-9 Upregulation During Prevention of Gastric Inflammation Induced by Ethanol in Rat
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
The use of Nano-Quercetin to Arrest Mitochondrial Damage and MMP-9 Upregulation During Prevention of Gastric Inflammation Induced by Ethanol in Rat
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| Creator |
Chakraborty, Somsuta
Stalin, Sami Das, Nirmalendu ThakurChoudhury, Somsubhra Ghosh, Swarupa Swarnakar, Snehasikta |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Gastric ulcer is a multifaceted process that involves reactive oxygen species (ROS) generation, extracellular
matrix degradation and mitochondrial damage. Mitochondria play a crucial role for homeostasis
of ROS and cell survival. In our study, we investigated the efficacy and mechanism of polymeric nanocapsuled
quercetin (NQC) over the free quercetin (QC) molecule in prevention of ethanol-induced gastric
ulcer in rat. NQC possessed significantly higher efficacy (w20 fold) than free QC while preventing gastric
ulcers. Our data show that prior administration of NQC and/or QC significantly blocked synthesis and
secretion of matrix metalloproteinase (MMP)-9 as well as infiltration of inflammatory cells and oxidative
damage in rat gastric tissues. As compared to free QC, NQC protected much better the mitochondrial
integrity and size along with mitochondrial functions by controlling succinate dehydrogenase and NADH
oxidase in rat gastric tissues. In addition, both free QC and NQC down regulated PARP-1 as well as
apoptosis during protection against ethanol-induced gastric ulcer. Herein, the effect of NQC was greater
than QC on expression of enzymes like cyclooxygenase and nitric oxidase synthase (NOS)-2.We conclude
that NQC with greater bioavailability offers significantly higher potency in downregulating MMP-9 and
NOS-2 as well as oxidative stress in blocking ethanol-induced gastric ulcer.
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| Publisher |
Elsevier
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1495/1/BIOMATERIALS__33_(10)_2991%2D3001;2012[34].pdf
Chakraborty, Somsuta and Stalin, Sami and Das, Nirmalendu and ThakurChoudhury, Somsubhra and Ghosh, Swarupa and Swarnakar, Snehasikta (2012) The use of Nano-Quercetin to Arrest Mitochondrial Damage and MMP-9 Upregulation During Prevention of Gastric Inflammation Induced by Ethanol in Rat. Biomaterials, 33 (10). pp. 2991-3001. |
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| Relation |
http://dx.doi.org/10.1016/j.biomaterials.2011.12.037
http://www.eprints.iicb.res.in/1495/ |
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