Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/8024/
JAFC-06-91 |
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| Title |
Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid.
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| Creator |
Sastry, M. C. S.
Narasinga Rao, M. S. |
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| Subject |
29 Protein Chemistry
Sunflower Seed |
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| Description |
Binding of chlorogenic acid by sunflower 11S protein and succinylated and N-ethylmaleimide-treated protein was measured at pH 4.0 in 0.1M acetate buffer. Succinylation reduced binding, whereas N-ethylmaleimide treatment did not. Analysis of the binding data showed that succinylation reduced the number of binding sites without affecting the binding affinity. N-Ethylmaleimide treatment reduced neither the number of binding sites nor the binding affinity. Succinylation dissociated the 11S protein, whereas N-ethylmaleimide treatment did not. The secondary structure of N-ethylmaleimide-treated protein was different from that of the unmodified protein.
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/8024/1/JAFC_1991_63-.pdf
Sastry, M. C. S. and Narasinga Rao, M. S. (1991) Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid. Journal of Agricultural and Food Chemistry, 39 (1). 63-66, 15 ref.. |
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