Studies on solubilization and stabilization of alpha pinene hydroxylase from Aspergillus niger for verbenol production.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/7579/
JFST-04-03 |
|
| Title |
Studies on solubilization and stabilization of alpha pinene hydroxylase from Aspergillus niger for verbenol production.
|
|
| Creator |
Nazhat ul, Ainn.
Renu, Agrawal |
|
| Subject |
04 Fungi
|
|
| Description |
A soil isolate of Aspergillus niger has been found to be able to convert alpha-pinene, a low cost substrate, to verbenol, a high value food flavouring. This study examined the solubilization and stabilization of alpha-pinene hydroxylase, a monooxygenase responsible for catalysing this bioconversion. It was demonstrated that the alpha-pinene hydroxylase, purified from the particulate fraction of an A. niger cell free extract, could be solubilized with sodium cholate, and stabilized with 30% sorbitol plus 5mM dithiothreitol. Furthermore, the enzyme was found to require NADPH as a cofactor for its activity.
|
|
| Date |
2003
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Rights |
—
|
|
| Identifier |
http://ir.cftri.com/7579/1/JSFT-2003_63-66.pdf
Nazhat ul, Ainn. and Renu, Agrawal (2003) Studies on solubilization and stabilization of alpha pinene hydroxylase from Aspergillus niger for verbenol production. Journal of Food Science and Technology, 40 (1). pp. 63-66. |
|