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Plackett-Burman design for determining the preference of Rhizomucor miehei lipase for FA in acidolysis reactions with coconut oil.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


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Relation	http://ir.cftri.com/7598/ JAOCS-19-02

Title	Plackett-Burman design for determining the preference of Rhizomucor miehei lipase for FA in acidolysis reactions with coconut oil.

Creator	Lokesh, B. R. Reena, Rao Divakar, S.

Subject	04 Fermentation Technology 19 Lipids-oils/fats

Description	Preference of Rhizomucor miehei lipase (EC 3.1.1.3) in the incorporation of 11 fatty acids (FA; C10:0 to C22:6) into coconut oil triacylglycerols (TAG) during acidolysis was studied by applying the Plackett-Burman experimental design. Enzymic acidolysis reactions were carried out in hexane at 37C for 48 h with coconut oil (0.1M) and a mixture of 11 FA at a TAG to FA molar ratio of 1:1. Lipase was used at the 5 wt.% level. Incorporation of FA into coconut oil TAG was determined by GC. The lipase showed preference for long-chain saturated FA for incorporation into coconut oil TAG. The FA with 18 C atoms showed a high incorporation rate (18:0 > 18:1 > 18:3). Lipase showed the lowest preference for incorporation of 12:0, which occurred at max. concn. (46%), whereas the most preferred FA, 18:0, occurred at a very low concn. (<less than or equal to> 2%) in coconut oil. The overall preference of lipase for incorporation of different FA into coconut oil TAG was 18:0 > 18:2, 22:0 >18:1, 18:3, 14:0, 20:4, 22:6 > 16:0 > 12:0 >> 10:0.

Date	2002

Type	Article PeerReviewed

Format	application/pdf

Language	en

Rights	—

Identifier	http://ir.cftri.com/7598/1/JAOCS%2C_Vol._79%2C_no._6_%282002%29.pdf Lokesh, B. R. and Reena, Rao and Divakar, S. (2002) Plackett-Burman design for determining the preference of Rhizomucor miehei lipase for FA in acidolysis reactions with coconut oil. Journal of the American Oil Chemists' Society, 79 (6). pp. 555-560.