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Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii)

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/9323/ FC-01-10

Title	Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii)

Creator	Harish Nayaka, M. A. Sathisha, U. V. Shylaja, M. Dharmesh

Subject	20 Plant Food 32 Antioxidants

Description	Swallow root (Decalepis hamiltonii) was extracted for free (SRFP), conjugated (SRCP) and insoluble-bound phenolic acids (SRIBP), and evaluated for cytoprotectivity, 1,1,diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, reducing power and protection to DNA damage. In addition, the constituent phenolic acids in the extracts were also analysed. Results indicated a total phenol content of 20.72, 7.97 and 11.52 mg gallic acid equivalents (GAE)/g for SRFP, SRCP and SRIBP extracts, respectively. At 0.12 lg/mL concentration SRCP showed 87% cytoprotection (on NIH 3T3 cells) compared to SRFP (47%) and SRIBP (65%). DPPH radical scavenging activity indicated an IC50 of 0.046, 0.06 and 0.128 lg/mL for SRCP, SRIBP and SRFP, respectively. Also, SRCP showed higher reducing power and DNA protectivity (80%). HPLC analysis of phenolic acid extracts showed the presence of hydroxybenzoate and cinnamate derivatives. Among the phenolics identified gallic, gentisic, protocatechuic and p-coumaric acids were the major contributors to antioxidant activity.

Date	2010

Type	Article PeerReviewed

Format	application/pdf

Language	en

Rights	—

Identifier	http://ir.cftri.com/9323/1/Food_Chemistry_2010_1307.pdf Harish Nayaka, M. A. and Sathisha, U. V. and Shylaja, M. Dharmesh (2010) Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii). Food Chemistry, 119. pp. 1307-1312.