Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii)
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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Relation |
http://ir.cftri.com/9707/
FC-06-10 |
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Title |
Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii)
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Creator |
Harish Nayaka, M. A.
Sathisha, U. V. Shylaja, M. Dharmesh |
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Subject |
10 Plants
32 Antioxidants |
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Description |
Swallow root (Decalepis hamiltonii) was extracted for free (SRFP), conjugated (SRCP) and insoluble-bound phenolic acids (SRIBP), and evaluated for cytoprotectivity, 1,1,diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, reducing power and protection to DNA damage. In addition, the constituent phenolic acids in the extracts were also analysed. Results indicated a total phenol content of 20.72, 7.97 and 11.52 mg gallic acid equivalents (GAE)/g for SRFP, SRCP and SRIBP extracts, respectively. At 0.12 μg/mL concentration SRCP showed 87% cytoprotection (on NIH 3T3 cells) compared to SRFP (47%) and SRIBP (65%). DPPH radical scavenging activity indicated an IC50 of 0.046, 0.06 and 0.128 μg/mL for SRCP, SRIBP and SRFP, respectively. Also, SRCP showed higher reducing power and DNA protectivity (80%). HPLC analysis of phenolic acid extracts showed the presence of hydroxybenzoate and cinnamate derivatives. Among the phenolics identified gallic, gentisic, protocatechuic and p-coumaric acids were the major contributors to antioxidant activity
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Date |
2010
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
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Identifier |
http://ir.cftri.com/9707/1/Food_Chemistry_119_2010_1307-1312.pdf
Harish Nayaka, M. A. and Sathisha, U. V. and Shylaja, M. Dharmesh (2010) Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii). Food Chemistry, 119 (4). pp. 1307-1312. |
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