Ascorbyl Stearate Promotes Apoptosis Through Intrinsic Mitochondrial Pathway in HeLa Cancer Cells.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/12739/
http://dx.doi.org/10.21873/anticanres.11238 |
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| Title |
Ascorbyl Stearate Promotes Apoptosis Through Intrinsic
Mitochondrial Pathway in HeLa Cancer Cells.
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| Creator |
Shirish, D. Mane
Maikho, Thoh Deepak, Sharma Santosh, K. Sandur Akhilender Naidu, K. |
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| Subject |
17 Vitamin Biochemistry
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| Description |
Background: Ascorbic acid is proposed to have
antitumor potential against certain cancer types but has the
limitation of requiring high doses for treating cancer.
Ascorbyl stearate (ASC-S) is a fatty acid ester derivative of
ascorbic acid with comparable potent apoptotic activity. The
present study was aimed at understanding the pathway
involved in apoptotic activity of ASC-S in cervical cancer
cells. Materials and Methods: The effect of ASC-S on
reactive oxygen species (ROS), and mitochondrial membrane
potential (MMP) was studied in HeLa cells. Furthermore,
the dose-dependent effect of ASC-S on release of cytochrome
c, pro-caspase-9, caspase-3, BH3 interacting-domain death
agonist (BID), truncated BH3 interacting-domain death
agonist (t-BID), FAS ligand (FASL) and transcription factors
nuclear factor-kappa B (NF-ĸB), nuclear factor of activated
T-cells (NFAT) and activator protein-1 (AP1) were studied in
HeLa cells. Results: Treatment of HeLa cells with ASC-S
significantly increased the MMP. The modulation of MMP
resulted in cleavage of BID, expression of FAS, cleavage of
pro-caspase-9 and release of cytochrome c into cytosol. In
addition, ASC-S treatment resulted in deregulation of
transcription factors NF-ĸB, NFAT and AP1, which play an
important role in the development of inflammation and
cancer. Conclusion: Our data, for the first time, suggest that
ASC-S has an apoptotic effect against HeLa cells by
inducing change in mitochondrial membrane permeability,
cytochrome c release and subsequent activation of caspase-
3 and NF-ĸB.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12739/1/ANTICANCER%20RESEARCH%2036%206409-6418%20%282016%29.pdf
Shirish, D. Mane and Maikho, Thoh and Deepak, Sharma and Santosh, K. Sandur and Akhilender Naidu, K. (2016) Ascorbyl Stearate Promotes Apoptosis Through Intrinsic Mitochondrial Pathway in HeLa Cancer Cells. Anticancer Research, 36. pp. 6409-6418. |
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