Celecoxib, a COX-2 inhibitor, synergistically potentiates the anti-inflammatory activity of docosahexaenoic acid in macrophage cell line.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive InfoField | Value | |
Relation |
http://ir.cftri.com/12743/
http://dx.doi.org/10.3109/08923973.2016.1147578 |
|
Title |
Celecoxib, a COX-2 inhibitor, synergistically potentiates the
anti-inflammatory activity of docosahexaenoic acid in
macrophage cell line.
|
|
Creator |
Vijay Kumar Reddy, K.
Akhilender Naidu, K. |
|
Subject |
11 Lipid Biochemistry
|
|
Description |
Background: The anti-inflammatory properties of eicosapentaenoic acid (EPA), docosahexaenoic
acid (DHA) and non-steroidal anti-inflammatory drugs overlap in many ways. The aim of this
study was to examine the individual and synergetic anti-inflammatory effects of celecoxib, EPA
and DHA in RAW-264.7 cell line.
Methodology: The cells were exposed to EPA, DHA, celecoxib, rosiglitazone, GW9662 alone or
their combination, and stimulated with 5 mg/mL lipopolysaccharide (LPS). Nitric oxide (NO),
tumor necrosis factor-a (TNF-a), interleukin (IL)-6 and prostaglandin-E2 (PGE2) levels were
estimated in the medium using enzyme-linked immunosorbent assays. The cyclooxygenase-2
(COX-2) and inducible Nitric Oxide Synthase (iNOS) expression were analyzed in the cell lysate
by immunoblotting. Peroxisome proliferator-activated receptor g (PPARg) and nuclear factor-kB
(NF-kB) transcription factor activation assays were performed in the nuclear extract.
Results: Combined treatment of DHA (50 mM) and celecoxib (20 mM) significantly inhibited LPS
induced synthesis of NO, TNF-a, IL-6 and PGE2 levels in the cells, compared to the individual
treatments. In addition, DHA and celecoxib diminished the COX-2 and iNOS expression in the
cells. This was associated with increased PPARg activity, supressed NF-kB activity in the nucleus.
We determined whether GW9662, a specific PPARg inhibitor, could abolish the antiinflammatory
effect of DHA and celecoxib. GW9662 has abolished the DHA and celecoxib
induced PPARg activation, but did not alter the NF-kB mediated anti-inflammatory effects
induced by celecoxib and DHA. Interestingly, EPA did not exhibit any inhibitory effect on these
parameters.
Conclusion: Our results suggest that DHA and celecoxib exhibit anti-inflammatory effect
through inhibition of NF-kB, independent of PPARg. Co-administration of celecoxib and DHA
would be promising approach for the treatment of inflammatory diseases.
|
|
Date |
2016
|
|
Type |
Article
PeerReviewed |
|
Format |
pdf
|
|
Language |
en
|
|
Identifier |
http://ir.cftri.com/12743/1/Immunopharmacol%20Immunotoxicol%2C%202016%3B%2038%282%29%20153%E2%80%93161.pdf
Vijay Kumar Reddy, K. and Akhilender Naidu, K. (2016) Celecoxib, a COX-2 inhibitor, synergistically potentiates the anti-inflammatory activity of docosahexaenoic acid in macrophage cell line. Immunopharmacology and Immunotoxicology, 38 (2). pp. 153-161. |
|