Standardized Bacopa monnieri extract ameliorates acute paraquat-induced oxidative stress, and neurotoxicity in prepubertal mice brain.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12746/
http://dx.doi.org/10.1179/1476830514Y.0000000149 |
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| Title |
Standardized Bacopa monnieri extract
ameliorates acute paraquat-induced oxidative
stress, and neurotoxicity in prepubertal mice
brain.
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| Creator |
Ravikumar, Hosamani
Gokul, Krishna Dr., Muralidhara |
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| Subject |
17 Toxicology
04 Medicinal Plants |
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| Description |
Objectives: Bacopa monnieri (BM), an ayurvedic medicinal plant, has attracted considerable interest owing
to its diverse neuropharmacological properties. Epidemiological studies have shown significant correlation
between paraquat (PQ) exposure and increased risk for Parkinson’s disease in humans. In this study, we
examined the propensity of standardized extract of BM to attenuate acute PQ-induced oxidative stress,
mitochondrial dysfunctions, and neurotoxicity in the different brain regions of prepubertal mice.
Methods: To test this hypothesis, prepubertal mice provided orally with standardized BM extract (200 mg/kg
body weight/day for 4 weeks) were challenged with an acute dose (15 mg/kg body weight, intraperitoneally)
of PQ after 3 hours of last dose of extract. Mice were sacrificed after 48 hours of PQ injection, and different
brain regions were isolated and subjected to biochemical determinations/quantification of central
monoamine (dopamine, DA) levels (by high-performance liquid chromatography).
Results: Oral supplementation of BM for 4 weeks resulted in significant reduction in the basal levels of
oxidative markers such as reactive oxygen species (ROS), malondialdehyde (MDA), and hydroperoxides
(HP) in various brain regions. PQ at the administered dose elicited marked oxidative stress within 48 hours
in various brain regions of mice. However, BM prophylaxis significantly improved oxidative homeostasis
by restoring PQ-induced ROS, MDA, and HP levels and also by attenuating mitochondrial dysfunction.
Interestingly, BM supplementation restored the activities of cholinergic enzymes along with the restoration
of striatal DA levels among the PQ-treated mice.
Discussion: Based on these findings, we infer that BM prophylaxis renders the brain resistant to PQ-mediated
oxidative perturbations and thus may be better exploited as a preventive approach to protect against
oxidative-mediated neuronal dysfunctions
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12746/1/Nutritional%20Neuroscience.pdf
Ravikumar, Hosamani and Gokul, Krishna and Dr., Muralidhara (2014) Standardized Bacopa monnieri extract ameliorates acute paraquat-induced oxidative stress, and neurotoxicity in prepubertal mice brain. Nutritional Neuroscience, 19 (10). pp. 434-446. |
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