Putrescine and polyamine inhibitors in culture medium alter in vitro rooting response of Decalepis hamiltonii Wight & Arn.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12753/
http://dx.doi.org/10.1007/s11240-016-1108-0 |
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| Title |
Putrescine and polyamine inhibitors in culture medium alter
in vitro rooting response of Decalepis hamiltonii Wight & Arn.
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| Creator |
Pradeep, Matam
Giridhar, P. |
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| Subject |
05 Tissue Culture
04 Medicinal Plants |
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| Description |
The influence of polyamine putrescine (PUT),
and polyamine inhibitors were tested for in vitro rooting
response from micro shoots that initially established on
Murashige and Skoog (MS) medium comprising 2.7 μM
α-Naphthaleneacetic acid (NAA) and 8.9 μM 6-Benzylaminopurine
(BA) by using nodal explants of Decalepis
hamiltonii. Incorporation of putrescine alone in rooting
medium devoid of auxins supported the best response for
in vitro rooting qualitatively and quantitatively. Incorporation
of putrescine at 50 μM able to induce 8.62 ± 1.93 roots
with a maximum root length of 9.10 ± 1.65 cm wherein, the
root fresh weight was also found to be high compared to
all other treatments (5.248 ± 1.71 g). Addition of putrescine
inhibitor cyclohexylamine (CHA) in medium curtailed rooting
response from microshoots. Among the three polyamine
inhibitors, CHA in presence of 9.8 μM Indole-3-butyric acid
(IBA) outperformed α-DL-difluromethylarginine (DFMA)
and α-DL-difluoromethylornithine (DFMO) combination
with 9.8 μM IBA. The least response for root number
(1.55 ± 0.72), root length (1.96 ± 0.45 cm), and root weight
(1.94 ± 0.35 g) was found for IBA + PUT + DFMA and the
best response was noted for IBA + PUT + CHA (2.6 ± 1.1,
2.92 ± 0.73 cm, 3.03 ± 0.75 g) respectively. Endogenous
content of putrescine, spermidine and spermine supported
the rooting response from in vitro shoots. These results
have clearly demonstrated that putrescine plays a crucial
role in rooting of D. hamiltonii. Plantlets were transferred to micro-pots for a short acclimatization stage in greenhouse
where they survived at 90 %. This highly reproducible
procedure can be adopted for large scale swallow root
propagation. Overall, supplementing putrescine in the rooting
medium enhances the quantity and quality of roots in
D. hamiltonii, thus confirming its role.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12753/1/Plant%20Cell%20Tiss%20Organ%20Cult%20%282017%29%20128273%E2%80%93282.pdf
Pradeep, Matam and Giridhar, P. (2017) Putrescine and polyamine inhibitors in culture medium alter in vitro rooting response of Decalepis hamiltonii Wight & Arn. Plant Cell Tissue and Organ Culture, 128. pp. 273-282. |
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