Squalene modulates fatty acid metabolism: Enhanced EPA/DHA in obese/diabetic mice (KK-Ay) model.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12758/
http://dx.doi.org/10.1002/ejlt.201600006 |
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| Title |
Squalene modulates fatty acid metabolism: Enhanced
EPA/DHA in obese/diabetic mice (KK-Ay) model.
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| Creator |
Sangeetha, Ravi Kumar
Yamauchi, I. Bhaskar, N. |
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| Subject |
17 Fatty Acid Chemistry
12 Metabolism |
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| Description |
Biosynthesis of long-chain n-3 fatty acids from precursors is limited. In vivo effect of squalene (SQ) on
the metabolic fate of n-3 fatty acid precursors in obese/diabetic KK-Ay rodent model was evaluated in our
work. Soybean oil, being rich in ALA (18:3 n-3; a known precursor of EPA/DHA), was chosen as the n-3
fatty acid precursor rich source. A high-fat diet (20%) containing 7% soybean oil (SO) was fed to obesity/
diabetes-prone male KK-Ay mice (control). In the case of diets fed to test groups, soybean oil was
replaced with 5% SO and 2% SQ. HepaticDHAlevels increased (four fold) in SQ fed group over control
(p<0.05). Gene and protein expressions of D5 and D6 desaturases, key enzymes involved in the fatty acid
metabolism, further supported the results. Also, SQ exhibited a hypotriglyceridemic and hypoglycemic
effect. The results clearly indicated the effect of SQ in modulating the n-3 fatty acid metabolism,
including EPA/DHA synthesis in the presence of n-3 fatty acid precursor. This is the first report of
enhancement of in vivo DHA/EPA by SQ and in turn, modulating the physiological fatty acid profile.
Practical applications: Squalene (SQ) is an important marine biofunctional material that is found in
some terrestrial sources as well. Squalene, being a cholesterol precursor, forms an interesting subject of
research for its effect in vivo. SQ significantly enhanced proportions of EPA and/or DHA when their n-3
fatty acid precursors were available in the diet. The study further establishes the usefulness of SQ in
functional food formulations. The work provides an important basis for further evaluation of the role of
SQ in normal and disease conditions.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12758/1/Eur.%20J.%20Lipid%20Sci.%20Technol.%202016%2C%20118%2C%201935%E2%80%931941.pdf
Sangeetha, Ravi Kumar and Yamauchi, I. and Bhaskar, N. (2016) Squalene modulates fatty acid metabolism: Enhanced EPA/DHA in obese/diabetic mice (KK-Ay) model. European Journal of Lipid Science and Technology, 118. pp. 1935-1941. |
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