Glucose-rich diet aggravates monocrotophos-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12761/
http://dx.doi.org/10.1002/jat.3426 |
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| Title |
Glucose-rich diet aggravates
monocrotophos-induced dopaminergic
neuronal dysfunction in Caenorhabditis elegans.
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| Creator |
Salim, Chinnu
Rajini, P. S. |
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| Subject |
26 Pesticide Chemistry
17 Toxicology |
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| Description |
The present study aimed to obtain insights into the mechanism(s) by which glucose-rich diet aggravates
monocrotophos (MCP)-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans. In this study, we exposed three
different strains of worms (wild-type N2, CB1112 (cat-2(e1112)II, tyrosine hydroxylase-deficient mutant, catecholamine absent)
and the transgenic BZ555 (egls1-dat-1p::green fluorescent protein [GFP]) (in which bright GFP is tagged to the dopamine neuronal
soma and processes) grown and maintained in normal nematode growthmedium or 2% glucose enriched-nematode growth
mediumtoMCP (0.75mM) for 48 h. After the exposure, dopamine-mediated behaviors such as repulsion to nonanone, chemotaxis
index and basal slowing response were determined in worms. Dopamine, 3,4-dihydroxy phenyl acetic acid and homovanillic acid
content were quantified in N2 worms. The extent of neurodegeneration was visualized and quantified in dat-1::GFPworms. Basal
slowing response study clearly indicated that cat-2 worms exposed to MCP and glucose were less affected compared to N2 of the
same treatment. Learning andmemory were affected byMCP and glucose.While MCP-treated worms showed lesser repulsion to
nonanone compared to control worms, MCP-treated, glucose-fed worms showed a greater reduction in repulsion to nonanone.
Further, MCP-treated, glucose-fed worms exhibited a marked reduction in dopamine content and an increase in 3,4-dihydroxy
phenyl acetic acid and homovanillic acid levels compared to that in control. Dat-1::GFP showed a significant degeneration of
dopaminergic neurons when exposed to glucose and MCP. Thus, our results clearly demonstrate that glucose-rich diet
aggravates the dopaminergic neuronal dysfunction induced by MCP in C. elegans.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12761/1/J.%20Appl.%20Toxicol.%202017%3B%2037%20772%E2%80%93780.pdf
Salim, Chinnu and Rajini, P. S. (2017) Glucose-rich diet aggravates monocrotophos-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans. Journal of Applied Toxicology, 37. pp. 772-780. |
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