CSIR Central

Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/12777/ http://dx.doi.org/10.1016/j.carbpol.2016.12.046

Title	Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.

Creator	Sabeeta, Kapoor Shylaja, M. Dharmesh

Subject	28 Polysaccharide Chemistry 03 Tomato

Description	tPectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and henceimpede cancer progression. We evaluated anticancer effect of Pectic-Oligosaccharide isolated from SourRaw Tomato (SrTPO); its bioavailability and structure elucidated from purified fraction (SrTPO1). SrTPO1inhibited galectin-3 activity with MIC of 0.25 ug/mL (100 fold better than standard galactose), inhibitedthe growth of AGS cells (IC503.4 �g/mL) and induced apoptosis (70% inhibition at 30 �g/mL concentra-tion). Normal– NIH 3T3 cells were not affected by SrTPO as opposed to doxorubicin, a known anticancerdrug, which reduced 76% viability at equivalent dose. SrTPO1 was identified as RhamnogalacturonanI-arabinogalactan (RGI-AG), where repeated alternative rhamnose and galacturonic acid residues wereobserved while arabinose in the branch point and �-1,4 linked galactose in the linear chain form. SrTPOwas found to be bioavailable as evaluated by FITC labelled oligos inside the cell, which was in reciprocalproportion with apoptosis.

Date	2017

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/12777/1/Carbohydrate%20Polymers%20160%20%282017%29%2052%E2%80%9361.pdf Sabeeta, Kapoor and Shylaja, M. Dharmesh (2017) Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship. Carbohydrate Polymers, 160. pp. 52-61.