Graphene–copper oxide nanocomposite with intrinsic peroxidase activity for enhancement of chemiluminescence signals and its application for detection of Bisphenol-A.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12828/
http://dx.doi.org/10.1016/j.snb.2016.02.017 |
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| Title |
Graphene–copper oxide nanocomposite with intrinsic peroxidase
activity for enhancement of chemiluminescence signals and its
application for detection of Bisphenol-A.
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| Creator |
Ragavan, K. V.
Rastogi, N. K. |
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| Subject |
07 Food Engineering
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| Description |
Graphene–copper(II) oxide (G–CuO) nanocomposite was synthesized for the first time by simple and
rapid microwave assisted co-precipitation method using copper acetate as a precursor. Synthesized
G–CuO nanocomposite showed the characteristic Raman and Fourier transform infrared peaks of CuO
below 700 cm−1. Characteristic crystalline peaks of CuO corresponding to monoclinic symmetry was
observed in X-ray diffraction analysis. Nanocomposite with quasi-spherical morphology was observed in
transmission electron microscopic analysis. Nanocatalytic activity of G–CuO nanocomposite was examined
by its efficiency to improve the signals of luminol–H2O2 chemiluminescence (CL) reaction. G–CuO
nanocomposite enhanced the signals of CL reaction by 280 times higher than control. Enhancement in CL
signals is attributed to intrinsic peroxidase like activity of G–CuO nanocomposite on H2O2 breakdown to
OH− and O2• ions. Formation of radicals and ions by nanocatalytic mechanism was hypothesized and confirmed
by trolox assay. G–CuO has higher intrinsic peroxidase like activity than bare copper oxide (CuO)
nanoparticles by 23 times. G–CuO nanocomposite can be a better alternative to peroxidases and nanomaterials
which are used to enhance the CL signals. Analytical application of enhanced luminol–H2O2
CL reaction was applied for detection of Bisphenol-A (BPA) in feeding bottles and polycarbonate water
bottles. Proposed enhanced CL method has linearity in the range 10,000–1 ng/mL with limit of detection
of 0.55 ng/mL and limit of quantification of 8.3 ng/mL. Intrinsic peroxidase like activity of synthesized
G–CuO can have wide application in nanocatalysis and analytical chemistry for the development of CL
based analytical and biomedical kits, sensors, and assays.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12828/1/Sensors%20and%20Actuators%20B%20229%20%282016%29%20570%E2%80%93580.pdf
Ragavan, K. V. and Rastogi, N. K. (2016) Graphene–copper oxide nanocomposite with intrinsic peroxidase activity for enhancement of chemiluminescence signals and its application for detection of Bisphenol-A. Sensors and Actuators B, 229. pp. 570-580. ISSN 0925-4005 |
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