Silver nitrate and 2-(N-morpholine) ethane sulphonic acid in culture medium promotes rapid shoot regeneration from the proximal zone of the leaf of Capsicum frutescens Mill.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13138/
http://dx.doi.org/10.1007/s11240-016-1157-4 |
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| Title |
Silver nitrate and 2-(N-morpholine) ethane sulphonic acid
in culture medium promotes rapid shoot regeneration
from the proximal zone of the leaf of Capsicum frutescens Mill.
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| Creator |
Ashwani, Sharma
Ravishankar, G. A. Giridhar, P. |
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| Subject |
05 Tissue Culture
30 Spices/Condiments |
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| Description |
The genetic manipulation of Capsicum has
been unsuccessful, and a large bottleneck to transferring the
desired genes is due to the difficulty in regenerating whole
plants through tissue culture because of its highly recalcitrant
and high genotype specificity. This study aimed to
investigate and establish rapid shoot regeneration from the
proximal ends of the leaves of Capsicum frutescens KT-OC
and BOX-RUB varieties. A maximum of 8–10 shoot buds
were obtained from the margins of the proximal portion of
a cotyledonary leaf explant of C. frutescens variety KT-OC
on medium I containing 44.44 μM 6-benzylaminopurine
(BA), 5.71 μM indole-3-acetic acid (IAA), 10 μM silver
nitrate (
AgNO3) and 1.98 mg L−1 2-(N-morpholine) ethane
sulphonic acid within 4 weeks of incubation, of which
60% of explants responded in terms of shoot buds. Petiole
explants (40%) cultured on the same medium produced 2–4
shoots per explant from the distal portion. The cut portions
of the cotyledonary leaf proximal portions responded well
to shoot bud formation in the presence of 22.20 μM BA
and 14.68 μM phenyl acetic acid (PAA), wherein 100% of
explants responded in terms of shoot bud formation, with
an average of 10 ± 1.7 and 8 ± 1.9 shoot buds per explant
in KT-OC and BOX-RUB varieties, respectively. The differentiated
shoots grew well and proliferated in the presence
of 14.68 μM PAA + 22.20 μM BA and 10 μM AgNO3.
Shoot elongation was obtained in presence of 1.44 μM gibberellic
acid (
GA3) and 10 μM AgNO3.
These shoots were
rooted on plant growth regulator-free half-strength MS medium and upon hardening; field survival rate was 70%.
This reproducible regeneration method for C. frutescens,
especially the Indian high pungent variety, from proximal
portion of cotyledonary leaf and petiole explants, can be
used for biotechnological improvement.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13138/1/Plant%20Cell%20Tiss%20Organ%20Cult%20%282017%29%20129175%E2%80%93180.pdf
Ashwani, Sharma and Ravishankar, G. A. and Giridhar, P. (2017) Silver nitrate and 2-(N-morpholine) ethane sulphonic acid in culture medium promotes rapid shoot regeneration from the proximal zone of the leaf of Capsicum frutescens Mill. Plant Cell Tissue and Organ Culture, 129. pp. 175-180. |
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