Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/13171/
http://dx.doi.org/10.3923/jm.2008.136.142 |
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| Title |
Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.
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| Creator |
Latha, R.
Manonmani, H. K. Rati, E. R. |
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| Subject |
09 Food Microbiology
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| Description |
Aflatoxins are potent secondary metabolites produced commonly by Aspergillus flavus, A. parasiticus and A. nomius. Primers were designed specifically for o-methyl transferase (omt) and aflatoxin regulatory gene (aflR) of aflatoxin biosynthetic pathway and also to detect the genus Aspergillus specific primers (18s rRNA genes) using NS. Experimental conditions were standardized for optimum multiplex PCR. DNA extracted from mycelia of toxigenic and non-toxigenic A. flavus, A parasiticus, other Aspergilli and from other genera of fungi were subjected to multiplex PCR using these primers. The omt and aflR primer pairs gave specific PCR amplification for aflatoxigenic A. flavus and A. parasiticus. They did not give DNA amplification for non-aflatoxigenic A. flavus, A. oryzae, A. glaucus, Fusarium, Penicillium and Rhizopus spp.
Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Available from:
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13171/1/136-142.pdf
Latha, R. and Manonmani, H. K. and Rati, E. R. (2008) Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Research Journal of Microbiology, 3 (3). pp. 136-142. |
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