Regeneration of NADPH Coupled with HMG-CoA Reductase Activity Increases Squalene Synthesis in Saccharomyces cerevisiae.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13315/
http://dx.doi.org/10.1021/acs.jafc.7b02945 |
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| Title |
Regeneration of NADPH Coupled with HMG-CoA Reductase Activity
Increases Squalene Synthesis in Saccharomyces cerevisiae.
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| Creator |
Kalaivani, P.
Sarma, Mutturi |
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| Subject |
07 Enzyme Biochemistry
02 Bacteriology |
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| Description |
Although overexpression of the tHMG1 gene is a well-known strategy for terpene synthesis in Saccharomyces
cerevisiae, the optimal level for tHMG1p has not been established. In the present study, it was observed that two copies of the
tHMG1 gene on a dual gene expression cassette improved squalene synthesis in laboratory strain by 16.8-fold in comparison to
single-copy expression. It was also observed that tHMG1p is limited by its cofactor (NADPH), as the overexpression of NADPH
regenerating genes’, viz., ZWF1 and POS5 (full length and without mitochondrial presequence), has led to its increased enzyme
activity. Further, it was demonstrated that overexpression of full-length POS5 has improved squalene synthesis in cytosol. Finally,
when tHMG1 and full-length POS5 were co-overexpressed there was a net 27.5-fold increase in squalene when compared to
control strain. These results suggest novel strategies to increase squalene accumulation in S. cerevisiae.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13315/1/J.%20Agric.%20Food%20Chem.%202017%2C%2065%2C%208162%E2%88%928170.pdf
Kalaivani, P. and Sarma, Mutturi (2017) Regeneration of NADPH Coupled with HMG-CoA Reductase Activity Increases Squalene Synthesis in Saccharomyces cerevisiae. Journal of Agricultural and Food Chemistry, 65. pp. 8162-8170. ISSN 0021-8561 |
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