Unravelling a stearidonic acidrich triacylglycerol biosynthetic pathway in the developing seeds of Buglossoides arvensis: A transcriptomic landscape.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13316/
http://dx.doi.org/10.1038/s41598-017-09882-y |
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| Title |
Unravelling a stearidonic acidrich
triacylglycerol biosynthetic
pathway in the developing
seeds of Buglossoides arvensis: A
transcriptomic landscape.
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| Creator |
Sreedhar, R. V.
Prasad, P. Prasanna Anjaneya Reddy, Lebaka Ram, Rajasekharan Malathi, Srinivasan |
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| Subject |
10 Plants
17 Fatty Acid Chemistry 01 Oilseeds |
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| Description |
potential applications in human health and nutrition. The molecular basis of SDA biosynthesis in this
plant remains unknown due to lack of genomic information. To unravel key genes involved in SDA-rich
triacylglycerol (TAG) biosynthesis, we performed transcriptome sequencing of pooled mRNA from
five different developmental stages of B. arvensis seeds using Illumina NextSeq platform. De novo
transcriptome assembly generated 102,888 clustered transcripts from 39.83 million high-quality reads.
Of these, 62.1% and 55.54% of transcripts were functionally annotated using Uniprot-Viridiplantae
and KOG databases, respectively. A total of 10,021 SSR-containing sequences were identified using
the MISA tool. Deep mining of transcriptome assembly using in silico tools led to the identification of
genes involved in fatty acid and TAG biosynthesis. Expression profiling of 17 key transcripts involved in
fatty acid desaturation and TAG biosynthesis showed expression patterns specific to the development
stage that positively correlated with polyunsaturated fatty acid accumulation in the developing seeds.
This first comprehensive transcriptome analysis provides the basis for future research on understanding
molecular mechanisms of SDA-rich TAG accumulation in B. arvensis and aids in biotechnological
production of SDA in other oilseed crops.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13316/1/ScIentIfIc%20Reports%20%207%2010473.pdf
Sreedhar, R. V. and Prasad, P. and Prasanna Anjaneya Reddy, Lebaka and Ram, Rajasekharan and Malathi, Srinivasan (2017) Unravelling a stearidonic acidrich triacylglycerol biosynthetic pathway in the developing seeds of Buglossoides arvensis: A transcriptomic landscape. Scientific Reports, 7. pp. 1-16. |
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