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Strain Improvement Through Mutagenesis and Optimization of Protease Production By Aspergillus Terreus Cjs-127 using Jatropha Seed Cake as Substrate.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/13325/ http://dx.doi.org/10.15414/jmbfs.2017.7.2.174-180

Title	Strain Improvement Through Mutagenesis and Optimization of Protease Production By Aspergillus Terreus Cjs-127 using Jatropha Seed Cake as Substrate.

Creator	Jyothna, B. Somashekar, D.

Subject	10 Plants 05 Enzymes 07 Waste utilization

Description	Jatropha curcas seedcake (JSC), a byproduct of the biodiesel industry was utilized for the production of protease. To enhance the protease production, the parent strain of Aspergillus terreus CJS-127 subjected to UV (15, 30, 60, 90, 120 and 150 min) and chemical mutagenesis by using ethyl methanesulphonate (EMS-125, 250 and 500 mg). Two mutant strains designated as UV-6 and EMS-5 selected for optimization of protease production using JSC medium by submerged fermentation. Under the optimized conditions of pH 7.0, temperature 30°C, incubation period 72 h, inoculum 105/ml spores, 150 rpm agitation, JSC at 2% level, the maximum protease production was 172.32 and 163.57 U/ml for EMS-5 and UV-6 mutant strains respectively. The addition of maltose (2%) was found to enhance the protease production in the mutant strains. The protease activity increased by 2.30 and 2.41 folds for UV 6 and EMS 5 mutant strains after optimization of cultural conditions.

Date	2017

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/13325/1/J%20Microbiol%20Biotech%20Food%20Sci%20%202017%20%207%20%282%29%20174-180.pdf Jyothna, B. and Somashekar, D. (2017) Strain Improvement Through Mutagenesis and Optimization of Protease Production By Aspergillus Terreus Cjs-127 using Jatropha Seed Cake as Substrate. Journal of Microbiology Biotechnology and Food Sciences, 7 (2). pp. 174-180.