Nano-engineering of liposomes by Supercritical CO2 mediated Gas Anti-solvent method.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13341/
http://dx.doi.org/10.1039/x0xx00000x |
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| Title |
Nano-engineering of liposomes by Supercritical CO2 mediated Gas
Anti-solvent method.
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| Creator |
Shashidhar, G. M.
Pravin, G. V. Manohar, B. |
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| Subject |
11 Lipid Biochemistry
02 Engineering & allied operations |
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| Description |
Nano-liposomes were designed by using Supercritical (SC) Gas Anti-solvent
(GAS) method. The present approach configured to combine two steps i,e 1)
SC−CO2 assisted drying of phospholipids to form uniform layer; 2) hydration
under pressure and depressurization of SC liquid phase to generate nanoliposomes.
Effect of pressure, temperature and surfactant concentration (Tween
80) was evaluated for production of nano-liposomes. SC−GAS method was
compared with Bangham’s method in terms of mean diameter, coefficient of
uniformity, morphology, stability index and actual energy required for liposomes
formation. Liposomes obtained by Bangham’s method were multi-lamellar
vesicles having coefficient of uniformity (12.07 ± 0.024) with mean diameter of
4.64 ± 0.010 μm, exhibiting irregular thickness of bilayer. Liposomes production
by SC−GAS method at optimized formulation conditions i,e 170 bar, 50 °C, 1.5%
Tween 80 and depressurization rate of 25 bar/min yielded nano-liposomes
exhibiting coefficient of uniformity (1.10 ± 0.012) with mean diameter of 0.063 ±
0.002 μm. Stability index and viscosity of prepared liposomes suspension have
indicated greater stability and near Newtonian behaviour respectively. SC−GAS
process requires 1.52 times lesser energy compared to Bangham’s method to
achieve nano-scale liposomes production. Storage studies indicated that SC−GAS
based nano-liposomes were highly stable up to 3 months at different temperatures.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13341/1/RSC%20Adv.%2C%202016%2C6%2C%2057739-57750.pdf
Shashidhar, G. M. and Pravin, G. V. and Manohar, B. (2016) Nano-engineering of liposomes by Supercritical CO2 mediated Gas Anti-solvent method. RSC Advances, 6 (62). pp. 57739-57750. |
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