Heterologous expression of β-carotene hydroxylase in Dunaliella salina by Agrobacterium-mediated genetic transformation.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13355/
http://dx.doi.org/10.1016/j.algal.2016.06.017 |
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| Title |
Heterologous expression of β-carotene hydroxylase in Dunaliella salina
by Agrobacterium-mediated genetic transformation.
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| Creator |
Daris, P. Simon
Anila, N. Gayathri, K. Sarada, R. |
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| Subject |
03 Biochemistry & Molecular Biology
01 Algae |
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| Description |
Dunaliella salina is a halotolerant marine microalga, known for the accumulation of β-carotene. The BCH-type β-
carotene hydroxylase plays a major role in the conversion of β-carotene to xanthophylls. The β-carotene hydroxylase
fromthe chlorophycean microalga, Chlamydomonas reinhardtii was amplified, and the sequence alignment
analysis showed that the amplified gene belongs to the group of BCH-type hydroxylases. The amplified β-carotene
hydroxylase gene with rubisco promoter and chloroplast transit peptide was introduced into the genome
of the D. salina by Agrobacterium-mediated genetic transformation. The carotenoid analysis showed a 3-fold increase
of violaxanthin at growth stage and a 2-fold increase of zeaxanthin under stress conditions in transformed
D. salina.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13355/1/Algal%20Research%2018%20%282016%29%20257%E2%80%93265.pdf
Daris, P. Simon and Anila, N. and Gayathri, K. and Sarada, R. (2016) Heterologous expression of β-carotene hydroxylase in Dunaliella salina by Agrobacterium-mediated genetic transformation. Algal Research, 18. pp. 257-265. |
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