Isolation and characterization of the major protein fraction of sunflower seeds.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/6329/
|
|
| Title |
Isolation and characterization of the major protein fraction of sunflower seeds.
|
|
| Creator |
Rahma, E. H.
Narasinga Rao, M. S. |
|
| Subject |
03 Proteins
Sunflower Seed |
|
| Description |
The major protein fraction in sunflower seeds was isolated in a form judged homogeneous by gel filtration, gel electrophoresis and ultracentrifugation. Sunflower meal was extracted with 10% NaCl solution, and after centrifugation the supernatant was fractionated by (NH4)2SO4 precipitation; the fraction precipitating between 6.5 and 13% saturation was dissolved in 1M NaCl and dialysed against 0.025M Tris-glycine buffer pH 8.3. Sedimentation value of the isolated protein was 11.6S; mol. wt. was 300 000. The protein consisted of at least 10 major subunits as judged by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The isolated 11.6S protein contain no P, 0.31% carbohydrate, 0.31% chlorogenic acid and 2 SH groups/300 000 mol. wt. unit. 1 SH group was normally not exposed to sulphydryl reagents in the native state. Information on molecular conformation from circular dichroism studies is also presented.
|
|
| Date |
1981
|
|
| Type |
Article
PeerReviewed |
|
| Format |
pdf
|
|
| Language |
en
|
|
| Identifier |
http://ir.cftri.com/6329/1/J.%20Agric.%20Food%20Chem.%20lQ81%2C%2029%2C%20518-521.pdf
Rahma, E. H. and Narasinga Rao, M. S. (1981) Isolation and characterization of the major protein fraction of sunflower seeds. Journal of Agricultural and Food Chemistry, 29 (3). 518-521, 19 ref.. |
|