Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/6365/
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| Title |
Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation.
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| Creator |
Padmanabhan, S.
Ramana Murthy, M. V. Lonsane, B. K. |
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| Subject |
03 Fungi
04 Fermentation Technology |
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| Description |
Aspergillus oryzae CFTRI 1480, an isolate from a spoiled moist sample of casein, produced 59 105 units of an extracellular proteinase/g dry mouldy bran (DMB) at 72 h in an arbitrarily formulated wheat bran medium in a solid state fermentation system. The enzyme production was significantly affected by mineral salt content and pH of the liquid used for moistening the wheat bran. Enzyme titres were enhanced 1.34-fold with the addition of 0.4% corn starch. Optimization of key parameters, i.e. initial moisture content, age and size of inoculum, increased the enzyme production to 191 869 units/g DMB and reduced fermentation time to 48 h. Such high titres in a simple medium, surpassing most of the literature reports, indicate the industrial importance of the culture. The properties of acetone-precipitated enzyme, viz. the optimum pH of 10.0, >95% activity between pH 7.0 and 10.0, temp. optimum at 55
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| Date |
1993
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/6365/1/Appl%20Microbiol%20Biotechnol%20%281993%29%2040499-503.pdf
Padmanabhan, S. and Ramana Murthy, M. V. and Lonsane, B. K. (1993) Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation. Applied Microbiology and Biotechnology, 40 (4). 499-503, 22 ref.. |
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