Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/5477/
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| Title |
Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus.
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| Creator |
Mohammad Kunhi, A. A.
Singh, R. |
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| Subject |
03 Fungi
05 Enzymes |
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| Description |
Screening of several fungal cultures resulted in the selection of an isolate of Aspergillus candidus
which produced a considerable amount of RNA-degrading enzyme in both surface and submerged methods
of cultivation. The conditions for the assay of the RNAase were standardized at pH 4.5, 55 ~ and using
0.25 ~o yeast RNA as substrate. The enzyme was stable at pit 5.2. EDTA was found to activate the enzyme
slightly. At temperatures 50-- 60 ~ there was considerable loss in enzyme activity which was traced to the
presence of a contaminating protease which presumably degraded the RNAase optimally at this temperature.
The protease could be preferentially inactivated at or above 75 ~ The crude enzyme, in addition to RNAaso
was found to possess DNAase, nonspecific phosphodiesterase and 3'- and 5'-phosphomonoesterase activities.
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| Date |
1981
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/5477/1/Folia%20Microbiol.%2026%2C%20328--333%20%281981%29.pdf
Mohammad Kunhi, A. A. and Singh, R. (1981) Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus. Folia Microbiologica, 26 (4). pp. 328-333. |
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