Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/4654/
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| Title |
Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase.
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| Creator |
Baskaran, N.
Prakash, V. Savithri, H. S. Radhakrishnan, A. N. Appaji Rao, N. |
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| Subject |
07 Enzyme Biochemistry
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| Description |
The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and
hydroxylamine with sheep liver serine hydroxymethyltransferase (EC 2.1.2.1) was studied by absorption
spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of 0-
amino-D-serine (OADS) with the enzyme [Baskaran, N., Prakash, V., Appu Rao, A. G., Radhakrishnan,
A. N., Savithri, H. S., & Appaji Rao, N. (1989) Biochemistry (preceding paper in this issue)]. The reaction
of AAA (0.5 mM) with the Schiff base of the enzyme resulted in the formation of pyridoxal 5'-phosphate
(PLP) and was biphasic with rate constants of 191 and 19 s-l. The formation of the PLP-AAA oxime
measured by decrease in absorbance at 388 nm on interaction of AAA with the enzyme had a rate constant
of 5.2 M-' s-l. On the other hand, the reaction of L-canaline with the enzyme was slower as measured by
the disruption of enzyme-Schiff base than the reaction of OADS and AAA. In contrast, the formation
of PLP as an intermediate could not be detected upon the interaction of hydroxylamine with the enzyme.
The reaction of D-cycloserine with the enzyme was much slower (1.6 X lo2 M-' s-l ) than the aminooxy
compounds. These observations indicate that the aminooxy compounds that are structural analogues of
serine (OADS, AAA, and canaline) formed PLP as an intermediate prior to the formation of oxime, whereas
with hydroxylamine such an intermediate could not be detected.
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/4654/1/Biochemistry%201989%2C%2028%2C%2096%2013-961%207.pdf
Baskaran, N. and Prakash, V. and Savithri, H. S. and Radhakrishnan, A. N. and Appaji Rao, N. (1989) Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase. Biochemistry, 28. pp. 9613-9617. |
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