Effect of chemical modification on structure and activity of glucoamylase from Aspergillus candidus and Rhizopus species.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/3852/
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| Title |
Effect of chemical modification on structure and activity of glucoamylase from Aspergillus candidus and Rhizopus species.
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| Creator |
Shenoy, B. C.
Appu Rao, A. G. Raghavendra Rao, M. R. |
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| Subject |
03 Fungi
05 Enzymes |
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| Description |
The histidine, tyrosine, tryptophan and carboxyl groups in the enzyme glucoamylase
from Aspergillus Candidus and Rhizopus species were modified using group specific reagents.
Treatment of the enzyme with diethylpyrocarbonate resulted in the modification of 0·3 and
1 histidine residues with only a slight loss in activity (10% and 35%) of glucoamylase from
Aspergillus candidus and Rhizopus species respectively. Modification of tyrosine either by Nacetylimidazole
or [I125]-leads to a partial loss of activity. Under denaturing conditions,
maltose did not help in protecting the enzyme against tyrosine modification or inactivation.
Treatment with 2-Hydroxy-5-nitro benzyl bromide in the presence of urea, photooxidation
at pH 9·0, N-bromosuccinamide at pH 4·8 resulted in a complete loss of activity· However,
the results of experiments in the presence of maltose and at pH 4·8 photooxidation and Nbromosuccinamide
treatment suggested the presence of two tryptophan residues at the
active site. There was a complete loss of enzyme activity when 10 and 28 carboxyl groups
from Aspergillus candidus and Rhizopus, respectively were modified. Modification in the
presence of substrate maltose, showed at least two carboxyl groups were present at the
active site of enzyme and that only one active center seems to be involved in breaking ally 3
types of α-glucosidic linkages namely α-1,4, α-1, 6 and α-l,3.
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/3852/1/J.%20Biosci.%2C%20Vol%C2%B7%2011%2C%20Numbers%201%E2%80%934%2C%20March%201987%2C%20pp%C2%B7%20339-350.pdf
Shenoy, B. C. and Appu Rao, A. G. and Raghavendra Rao, M. R. (1987) Effect of chemical modification on structure and activity of glucoamylase from Aspergillus candidus and Rhizopus species. Journal of Biosciences, 11 (1-4). pp. 339-350. |
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