Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/2711/
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| Title |
Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed.
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| Creator |
Mohan Reddy, I.
Narasinga Rao, M. S. |
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| Subject |
03 Proteins
03 Cotton seed |
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| Description |
A method for the isolation of gossypin and congossypin from glanded cottonseed has been developed
on the basis of their solubility characteristics as a function of ionic strength and temperature. Further
purification of the proteins by gel filtration on Sephadex G-200 column yielded homogeneous (-98%)
preparations, as judged by sedimentation velocity, gel electrophoresis, and ion-exchange chromatography.
Gossypin and congossypin were characterized by sedimentation constants (S20,wof) 11.0 and 7.6 and
E;& at 280 nm of 7.6 and 6.0, respectively. The proteins were free from gossypol (free and bound) and
nucleic acid impurities.
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| Date |
1988
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2711/1/Journal%20of%20Agricultural%20and%20Food%20Chemistry%2C%20Volume-36%282%20%281988%29%20237-240.pdf
Mohan Reddy, I. and Narasinga Rao, M. S. (1988) Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed. Journal of Agricultural and Food Chemistry, 36 (2). pp. 237-240. |
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