Partial specific volume and interaction of glycinin with solvent components in urea and guanidine hydrochloride.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/2835/
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| Title |
Partial specific volume and interaction of glycinin with solvent components in urea and guanidine hydrochloride.
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| Creator |
Suresh Chandra, B. R.
Prakash, V. Narasinga Rao, M. S. |
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| Subject |
03 Proteins
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| Description |
The interaction of glycinin with urea/guanidine hydrochloride was investigated
by determining the apparent partial specific volume of the protein in these
solvents. The apparent partial specific volume was determined under both
isomolal and isopotential conditions. The preferential interaction parameter with
solvent components had values of 0.1 0 and 0.1 1 g urea and guanidine hydrochloride
respectively per g of protein. In both the cases the interaction was not
preferential with water. The total binding of urea/guanidine hydrochloride to
glycinin was calculated. Excellent correlation between the calculated value and
the total number of peptide bonds and aromatic amino acids residues of protein
was observed. The changes in volume upon transferring glycinin from dilute salt
solution to urea and guanidine hydrochloride solution were also calculated.
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| Date |
1986
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2835/1/Int.%20J.%20Peptide%20Protein%20Res.%2027%2C%201986%2C%20138-144.pdf
Suresh Chandra, B. R. and Prakash, V. and Narasinga Rao, M. S. (1986) Partial specific volume and interaction of glycinin with solvent components in urea and guanidine hydrochloride. International Journal of Peptide and Protein Research, 27. pp. 138-144. |
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