A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/2275/
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| Title |
A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.
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| Creator |
Shankar, C. S.
Umesh Kumar, S. |
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| Subject |
04 Yeast
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| Description |
A cell-wall-surface protein purified from the cells of Saccharomyces cerevisiae NCYC 227 was found to be involved in the non-sexual flocculation of this yeast. This 13 kDa protein was found to bind specifically to mannose. The protein bound to mannans isolated from yeast as well as in situ to intact cells, but only in the presence of calcium ions. The protein, a mannoprotein, formed aggregates as revealed in SDS-PAGE. Urea and higher temperatures prevented protein aggregation, suggesting that the flocculation of S. cerevisiae is primarily due to hydrogen bonding between mannan and protein.
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| Date |
1994
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2275/1/Microbiology%20%281%20994%29%2C%20140%2C%201097-1%20101.pdf
Shankar, C. S. and Umesh Kumar, S. (1994) A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae. Microbiology, 140. pp. 1097-101. ISSN 1350-0872 |
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