Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/1993/
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| Title |
Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography.
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| Creator |
Lakshminarayana, Rangaswamy
Raju, Marisiddaiah Krishnakantha, Thirumalai Parthasarathy Baskaran, V. |
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| Subject |
14 Carotenoid Chemistry
06 Chromatography 23 Vegetables |
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| Description |
Leafy vegetables [Basella rubra L., Peucedanum sowa Roxb., Moringa oleifera Lam., Trigonella foenum-graecum L., Spinacia oleracea L., Sesbania grandiflora (L.) Poir., and Raphanus sativus L.] that are commonly used by the rural population in India were evaluated in terms of their main carotenoid pattern. The extracted carotenoids were purified by open column chromatography (OCC) on a neutral alumina column to verify their identity by their characteristic UV-visible absorption spectra. Reverse-phase high-performance liquid chromatography (HPLC) on a C18 column with UV-visible photodiode array detection under isocratic conditions was used for quantification of isolated carotenoids. Acetonitrile/methanol/dichloromethane (60:20:20 v/v/v) containing 0.1% ammonium acetate was used as a mobile phase. The major carotenoids identified by both methods were lutein, beta-carotene, violaxanthin, neoxanthin, and zeaxanthin. Among the carotenoids identified, lutein and beta-carotene levels were found to be higher in these leafy vegetables. Results show that P. sowa and S. oleracea are rich sources of lutein (77-92 mg/100 g of dry wt) and beta-carotene (36-44 mg/100 g of dry wt) compared with other leafy vegetables. The purity of carotenoids eluted by OCC was clarified by HPLC, and they were found to be 92% +/- 3% for neoxanthin, 94% +/- 2% for violaxanthin, 97% +/-2% for lutein and zeaxanthin, and 90% +/- 3% for beta-carotene. It could be recommended to use P. sowa and S. oleracea as rich sources of lutein and beta-carotene for health benefits. The OCC method proposed is relatively simple and provides purified carotenoids for feeding trials.
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| Date |
2005
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Article
PeerReviewed |
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pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/1993/1/J.%20Agric.%20Food%20Chem.%202005%2C%2053%2C%202838-2842.pdf
Lakshminarayana, Rangaswamy and Raju, Marisiddaiah and Krishnakantha, Thirumalai Parthasarathy and Baskaran, V. (2005) Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 53 (8). pp. 2838-42. ISSN 0021-8561 |
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